What are Murashige and Skoog MS salts and what are some of the ingredients that are found in them?

Ingredients

• Ammonium nitrate (NH4NO3) 1650 mg/l.
• Calcium chloride (CaCl2 · 2H2O) 440 mg/l.
• Magnesium sulfate (MgSO4 · 7H2O) 370 mg/l.
• Monopotassium phosphate (KH2PO4) 170 mg/l.
• Potassium nitrate (KNO3) 1900 mg/l.

What is ms basal medium?

General description. Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators.

What is Murashige and Skoog medium used for?

Murashige and Skoog medium (or MSO or MS0 (MS-zero)) is a plant growth medium used in the laboratories for cultivation of plant cell culture. MS medium was originally formulated by Murashige and Skoog in 1962 to optimize tobacco callus bioassay system for facilitating the study of cytokinins.

What is the PH of MS medium?

The most commonly used culture medium is the Murashige and Skoog (MS) medium (Murashige and Skoog, 1962) or the variant of it. The pH of the medium is generally suggested as 5.5–6.0 and not much research on it has been reported (Beyl, 2011).

How do you adjust the pH of a medium?

Adjusting medium pH is normally done by adding diluted HCl or NaOH solution. In many cases, repeated adjustment by adding HCl and NaOH in turn is needed to get an exact pH value.

What’s the difference between Murashige and Skoog media?

Note:Linsmaier & Skoog (LS) Medium contains the same inorganic salts as Murashige & Skoog (MS) Medium. LS Medium differs from MS Medium ONLY in the vitamin content. The composition of vitamins in each basal medium is noted after the MS -based media table on the following pages. Phyto Technology Laboratories®

What to do if you ingest Murashige or Skoog?

Rinse opened eye immediately with running water, also wash under the eyelids, for at least 15 minutes. Seek medical advice immediately. IngestionRinse out mouth with water, then drink plenty of water. Do not induce vomiting. Seek medical help immediately. Self-protection of the first aiderRinse your hands with water after handling the powder.

What’s the difference between linsmaier and Skoog medium?

Linsmaier & Skoog (LS) Medium contains the same inorganic salts as Murashige & Skoog (MS) Medium. LS Medium differs from MS Medium ONLY in the vitamin content.

Is the Murashige medium too salty for plants?

Due to the high concentration of minerals, MS medium is a very rich and -saline -medium and may be too salty to certain plant species. To avoid this problem, MS is often used with the micro elements in full concentration, but with the macro elements in respectively half or three-quarter of the concentration as originally described by the authors.