How do you make BSA standards?
How do you make BSA standards?
Mix 50 μl of the BSA standard solution (2 mg/ml) with 950 μl of diluent and mix well to prepare a 0.1 mg/ml BSA standard solution. Prepare 2 sets of the dilutions of BSA standard solution as shown below 1.5 ml microtubes (7 types x 2 sets = 14 microtubes).
How do you dilute albumin standards?
A. Dilute the contents of one Albumin Standard (BSA) ampule into several clean vials, preferably using the same diluent as the sample(s). Each 1 ml ampule of 2.0 mg/ml Albumin Standard is sufficient to prepare a set of diluted standards for either working range suggested in Table 1.
What is the Pierce BCA protein assay based on?
The Thermo Scientific™ Pierce™ BCA Protein Assay is a detergent-compatible formulation based on bicinchoninic acid (BCA) for the colorimetric detection and quantitation of total protein.
What is albumin standard?
The albumin standard is precisely formulated at 2 mg/mL in an ultrapure 0.9% sodium chloride (saline) solution. The concentration of the stock solution is calibrated by direct comparison to purified BSA (Fraction V) from the National Institute of Standards and Technology (NIST).
How will you prepare a 5% BSA solution?
For example, to make a 100mL 3% BSA solution, use 3 grams of BSA and bring the volume to 100mL. To make a 5% solution of BSA in 500mL of media (which is the typical media bottle size) 25grams of BSA would be used, brought to a volume of 500mL with the media of interest.
Why is BSA used for standard curves?
BSA is used because of its stability to increase signal in assays, its lack of effect in many biochemical reactions, and its low cost, since large quantities of it can be readily purified from bovine blood, a byproduct of the cattle industry.
How do you make a 5 BSA solution?
Popular Answers (1) For example, to make a 100mL 3% BSA solution, use 3 grams of BSA and bring the volume to 100mL. To make a 5% solution of BSA in 500mL of media (which is the typical media bottle size) 25grams of BSA would be used, brought to a volume of 500mL with the media of interest.
How do you make a 10 BSA solution?
To prepare a solution of 10% BSA in NPB, dissolve 2 g of BSA in 15 mL of 1× NPB. Adjust the pH to 7.6 with KOH, and bring the volume to 20 mL with 1× NPB.
What are BSA standards?
BSA Standards are high-quality reference samples for generating accurate standard curves and calibration controls in total protein assays. The bovine serum albumin (BSA) solution is protein concentration reference standards for use in BCA, Bradford and other protein assay protocols.
How is albumin measured?
Albumin is a protein made by the liver. A serum albumin test measures the amount of this protein in the clear liquid portion of the blood. Albumin can also be measured in the urine .
How to order Thermo Scientific Pierce BSA protein assay?
Order requests are processed and shipped by your local distributor. Learn more Thermo Scientific Pierce BSA Protein Assay Standards are high-quality reference samples for generating accurate standard curves and calibration controls in total protein assays.
What are the BSA standard ampules for Pierce?
The official standard is of traceable origin with an identification number of NIST # 927. Our specifications require the BSA concentration to be 2.00 ± 0.03 mg/mL. How should I open the glass BSA standard ampules?
How is the Pierce BSA protein digest produced?
The Pierce BSA Protein Digest Standard is produced using high quality bovine serum albumin (BSA) and mass spectrometry-grade trypsin. The BSA is fully reduced, alkylated (iodoacetamide), and desalted (C18) after trypsin digestion to provide a quality LC/MS-grade standard that is free of intact protein.
What are the challenges of diluted BSA standards?
The challenge of diluted BSA standards is that they need to be protected from microbial growth, evaporation and protein precipitation. These will result in artificially high or low protein concentration values.