What is the recognition sequence for PvuII?

The PvuII REase (R · PvuII) recognizes the sequence CAGCTG and cleaves the central GpC on both strands to yield blunt ends (24); this enzyme has been crystallographically characterized as an apoenzyme and in complex with its DNA substrate (8, 19, 30).

What is the recognition site for HindIII?

Thermo Scientific HindIII restriction enzyme recognizes A^AGCTT sites and cuts best at 37°C in R buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes.

What is the recognition sequence for PstI?

PstI is a restiction enzyme used in molecular biology applications to cleave DNA at the recognition sequence 5′-CTGCA/G-3′, generating DNA fragments with 3′- cohesive termini.

Which enzyme can link two DNA fragments together?

ligases
In cells and in the lab, enzymes called ligases are used to join fragments of DNA together. Only DNA fragments that have matching, complementary ends can be joined by ligation.

What is the optimum temperature for most restriction enzyme reactions?

37°C.
The optimum temperature for the activity of most restriction enzymes is 37°C. Some enzymes, however, work best at 55°C, 65°C, or even 75°C! That’s because these enzymes are derived from extremophiles, microorganisms that live at very high temperatures under extreme conditions.

Which is the high fidelity version of PvuII?

Product Information PvuII has a High Fidelity version PvuII-HF ® (NEB #R3151). High Fidelity (HF) Restriction Enzymes have 100% activity in CutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity.

Which is the terminal branch of the SMA?

The SMA terminates at the ileum where it anastomoses with the ileal branch of the ileocolic artery. Some anatomists believe the ileocolic artery is the terminal portion of the SMA and that what many consider the terminal SMA is the last of the ileal branches. The superior mesenteric vein (SMV) should be positioned to the right of the SMA.

How many units of DNA does PvuII cut?

One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl. May exhibit star activity in CutSmart Buffer. PvuII does not cut CAGm4CTG, while cutting at CAGm5CTG occurs at a significantly reduced rate.

Is it normal to reverse the SMA / SMV relationship?

Reversal of SMA/SMV relationship is classically associated with intestinal malrotation, although distally (>6 cm from origin) this may be a normal finding 6.