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What does high fluorescence anisotropy mean?

What does high fluorescence anisotropy mean?

Fluorescence anisotropy or fluorescence polarization is a measurement of the changing orientation of a molecule in space, with respect to the time between the absorption and emission events. IVH indicates the intensity when using a vertical polarizer on the excitation and horizontal polarizer on the emission.

How do you calculate fluorescence anisotropy?

Fluorescence anisotropy is defined by(12)r=I∥−I⊥I∥+2I⊥where I‖ and I⊥ are the fluorescence intensities of the vertically (I‖) and horizontally (I⊥) polarized emission, when the sample is excited with vertically polarized light (Lakowicz, 1999).

What is rhodamine fluorescence?

Rhodamine B is a xanthene dye, which functions as a water tracer fluorescent. It is used as a staining fluorescent dye.

How do you quench fluorescence?

Fluorescence quenching refers to any process that decreases the fluorescence intensity of a sample. A variety of molecular interactions can result in quenching. These include excited-state reactions, molecular rearrangements, energy transfer, ground-state complex formation, and colli-sional quenching.

What is the difference between rhodamine B and rhodamine 6G?

B offers a high photostable and efficient laser dye in sol–gel silica sensitized with Rh. 6G; 75,000 shots as a laser half-lifetime of the sample and 24% efficiency at pumping intensity 0.1 J/cm2 of 532 nm. Wavelength shift occurs from 606 to 630 nm in the Rh.

What are anisotropics?

Anisotropic is the property of obtaining different values when observing or measuring something from different directions. The converse, isotropy, implies identical properties in all directions. Anisotropic properties of a material include its refractive index, tensile strength, absorbency, etc.

How is Rhodamine 6G used in dye lasers?

Rhodamine 6G (R6G), also known as Rhodamine 590, is one of the most frequently used dyes for application in dye lasers and as a fluorescence tracer, e.g., in the area of environmental hydraulics. Knowing the spectroscopic characteristics of the optical emission is key to obtaining high conversion efficiency and measurement accuracy, respectively.

What are the fluorescence fluctuations of rhodamine 6G?

The intensity fluctuations were in the order of 2% and the shape (confirmed by a comparison of the normalized spectra) was constant over time. This does not only indicate good repeatability but also exclude effects such as photo-bleaching as a result of illuminating the dye molecules with laser light.

How is rhodamine 590 used as a lasing medium?

Rhodamine 6G (R6G), also known as Rhodamine 590, is widely used as a lasing medium and as a fluorescence tracer [7]. The chemical structure of the R6G molecule is illustrated in Fig. 1. For use in dye lasers, it is dissolved in methanol, ethanol and a variety of other organic solvents.

What is the concentration of rhodamine 6G in ethanol?

The chemical structure of the R6G molecule is illustrated in Fig. 1. For use in dye lasers, it is dissolved in methanol, ethanol and a variety of other organic solvents. In environmental flow studies, the tracer medium is typically water. In ethanol, the absorption of R6G ranges between 440 nm and 570 nm, with the peak at 530 nm [7].