What antibiotic resistance does the pUC19 plasmid carry?

Plasmids pT7-7 and pUC19 carry an ampicillin-resistance gene (bla), pBR322 carries ampicillin- (bla) and tetracycline-resistance (tetA) genes, pCattTrE18 carries a chloramphenicol-resistance gene (cat), pACYC184 carries tetracycline- (tetA) and chloramphenicol-resistance (cat) genes, pACYC177 carries ampicillin- (bla) …

Is pUC19 ampicillin resistant?

The pUC19 plasmid confers ampicillin resistance to its host through its bla gene which codes for a β-lactamase that cleaves the β-lactam ring in ampicillin. The pUC19 plasmid also contains the lacZ gene which encodes the N-terminal fragment of the enzyme beta-galactosidase.

What is pUC19 plasmid used for?

pUC19 is a commonly used cloning vector that conveys the Amp resistance. The molecule is a small double-stranded circle, 2686 base pairs in length, and has a high copy number.

Which antibiotic resistance is present in pBR322?

pBR322 is 4361 base pairs in length and has two antibiotic resistance genes – the gene bla encoding the ampicillin resistance (AmpR) protein, and the gene tetA encoding the tetracycline resistance (TetR) protein.

What are the advantages of pBR322 as a cloning vector?

pBR322 has a better origin of replication, giving more DNA per cell. There are many more sites in antibiotic resistance genes for more cloning uses. The two markers in pBR322 are better for finding insert-containing clones by plating on one media, then transferring to another. pBR322 is also a smaller vector.

How is the pUC19 plasmid used in E coli?

pUC19 is a commonly used plasmid cloning vector in E.coli. The molecule is a double-stranded circular DNA (2686 base pairs in length). The pUC19 plasmid confers ampicillin resistance and complement defects in β-galactosidase in appropriate host strains.

How many base pairs are in the vector pUC19?

pUC19 Vector. The molecule is a small double-stranded circle, 2686 base pairs in length, and has a high copy number. pUC19 carries a 54 base-pair multiple cloning site polylinker that contains unique sites for 13 different hexanucleotide-specific restriction endonucleases (1).

What kind of polylinker does pUC19 have?

pUC19 carries a 54 base-pair multiple cloning site polylinker that contains unique sites for 13 different hexanucleotide-specific restriction endonucleases (1). NEB offers a selection of common cloning plasmids and DNAs for use as substrates. DNASU and Addgene are central repositories for plasmid clones and collections that may also be helpful.

How are plasmids used to develop antibiotic resistance?

Plasmids 101: Antibiotic Resistance Genes. Resistance to antibiotics is a widely used tool in molecular biology, yet scientists rarely stop to think about how much easier it makes our lives. Plasmid transformation into E. coli is a fairly inefficient process– just 1 out of 10,000 cells on average!